Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/9556
Title: Flow-through colorimetric assay for detection of nucleic acids in plasma
Authors: Ammanath, Gopal
Yeasmi, Sanjida
Srinivasulu, Yuvasri
Vats, Mukti
Cheema, Jamal Ahmed
Nabilah, Fairuz
Liedberg, Bo
Yıldız, Ümit Hakan
Yıldız, Ümit Hakan
Izmir Institute of Technology. Chemistry
Keywords: Colorimetric array
Polythiophene
Plasma
Nucleic acids
mir21
HBV-DNA
Issue Date: 2019
Publisher: Elsevier Ltd.
Abstract: A flow-through colorimetric assay for detection of nucleic acids in plasma is reported. The proposed assay features an array of four polyvinylidene fluoride (PVDF) membranes impregnated with cationic poly (3-alkoxy-4-methylthiophene) (PT) as an optical reporter. The sensing strategy is based on monitoring the changes in optical properties of PT, upon complexation with target nucleic acids in the presence and in the absence of their corresponding complementary peptide nucleic acids (PNAs). As a proof of concept, the proposed methodology is validated using two biomarkers; lung cancer associated microRNA (mir21) and hepatitis B virus DNA (HBV-DNA). The flow-through colorimetric assay enabled detection of mir21 and HBV-DNA in plasma without requiring tedious sample pre-treatment and clean up protocols. Colorimetric responses for mir21 and HBV-DNA were obtained at nanomolar concentrations over five orders of magnitudes (from 1 nM to 10 mu M), with a limit of detection of -0.6 nM and -2 nM in DI water and plasma, respectively. A logic gate system was developed to utilize the colorimetric assay responses as inputs for discrimination of mir21 and HBV-DNA and subsequently to obtain a profile of nucleic acids in samples that exceed respective clinical threshold limits, thereby enabling rapid and point of care (POC) disease diagnosis. Furthermore, the proposed methodology can be utilized for detection of a large number of nucleic acids in plasma by extending the array of PT impregnated membranes incorporated with their corresponding complementary PNAs. (C) 2019 Elsevier B.V. All rights reserved.
Description: WOS: 000465212100011
PubMed: 31027524
URI: https://doi.org/10.1016/j.aca.2019.03.036
https://hdl.handle.net/11147/9556
ISSN: 0003-2670
1873-4324
Appears in Collections:PubMed İndeksli Yayınlar Koleksiyonu / PubMed Indexed Publications Collection
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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