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dc.contributor.authorÖztürk, Saniye Elvan
dc.contributor.authorGöktay, Mehmet
dc.contributor.authorHas, Canan
dc.contributor.authorBabaoğlu, Mehmet
dc.contributor.authorAllmer, Jens
dc.contributor.authorDoğanlar, Sami
dc.contributor.authorFrary, Anne
dc.date.accessioned2020-01-08T12:09:58Z
dc.date.available2020-01-08T12:09:58Z
dc.date.issued2018-05en_US
dc.identifier.citationÖztürk, S. E., Göktay, M., Has, C., Babaoğlu, M., Allmer, J., Doğanlar, S., and Frary, A. (2018). Transcriptomic analysis of boron hyperaccumulation mechanisms in Puccinellia distans. Chemosphere, 199, 390-401. doi:10.1016/j.chemosphere.2018.02.070en_US
dc.identifier.issn0045-6535
dc.identifier.urihttps://doi.org/10.1016/j.chemosphere.2018.02.070
dc.identifier.urihttps://hdl.handle.net/11147/7573
dc.description.abstractPuccinellia distans, common alkali grass, is found throughout the world and can survive in soils with boron concentrations that are lethal for other plant species. Indeed, P. distans accumulates very high levels of this element. Despite these interesting features, very little research has been performed to elucidate the boron tolerance mechanism in this species. In this study, P. distans samples were treated for three weeks with normal (0.5 mg L−1) and elevated (500 mg L−1) boron levels in hydroponic solution. Expressed sequence tags (ESTs) derived from shoot tissue were analyzed by RNA sequencing to identify genes up and down-regulated under boron stress. In this way, 3312 differentially expressed transcripts were detected, 67.7% of which were up-regulated and 32.3% of which were down-regulated in boron-treated plants. To partially confirm the RNA sequencing results, 32 randomly selected transcripts were analyzed for their expression levels in boron-treated plants. The results agreed with the expected direction of change (up or down-regulation). A total of 1652 transcripts had homologs in A. thaliana and/or O. sativa and mapped to 1107 different proteins. Functional annotation of these proteins indicated that the boron tolerance and hyperaccumulation mechanisms of P. distans involve many transcriptomic changes including: alterations in the malate pathway, changes in cell wall components that may allow sequestration of excess boron without toxic effects, and increased expression of at least one putative boron transporter and two putative aquaporins. Elucidation of the boron accumulation mechanism is important in developing approaches for bioremediation of boron contaminated soils.en_US
dc.description.sponsorshipTUBITAK (113Z930)en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/j.chemosphere.2018.02.070en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBioremediationen_US
dc.subjectBoronen_US
dc.subjectHyperaccumulationen_US
dc.subjectPuccinellia distansen_US
dc.subjectRNA sequencingen_US
dc.titleTranscriptomic analysis of boron hyperaccumulation mechanisms in Puccinellia distansen_US
dc.typearticleen_US
dc.contributor.authorID0000-0002-5712-0826en_US
dc.contributor.authorID0000-0002-8973-0100en_US
dc.contributor.iztechauthorÖztürk, Saniye Elvan
dc.contributor.iztechauthorGöktay, Mehmet
dc.contributor.iztechauthorHas, Canan
dc.contributor.iztechauthorAllmer, Jens
dc.contributor.iztechauthorDoğanlar, Sami
dc.contributor.iztechauthorFrary, Anne
dc.relation.journalChemosphereen_US
dc.contributor.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume199en_US
dc.identifier.startpage390en_US
dc.identifier.endpage401en_US
dc.identifier.wosWOS:000428973200047
dc.identifier.scopusSCOPUS:2-s2.0-85042233972
dc.relation.tubitakinfo:eu-repo/grantAgreement/TUBITAK/KBAG/113Z930
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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