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dc.contributor.authorGüleç, Afife
dc.contributor.authorGüleç, Şükrü
dc.date.accessioned2019-02-21T07:46:26Z
dc.date.available2019-02-21T07:46:26Z
dc.date.issued2018-09
dc.identifier.citationGüleç, A., and Güleç, Ş. (2018). Ankaferd influences mRNA expression of iron-regulated genes during iron-deficiency anemia. Clinical and Applied Thrombosis/Hemostasis, 24(6), 960-964. doi:10.1177/1076029617737838en_US
dc.identifier.issn1076-0296
dc.identifier.urihttps://doi.org/10.1177/1076029617737838
dc.identifier.urihttp://hdl.handle.net/11147/7124
dc.description.abstractAnkaferd Blood Stopper (ABS) comprises a mixture of plants and stops bleeding via forming a protein network by erythroid aggregation. Bleeding causes reduction of iron levels in body. It has been indicated that ABS contains significant amount of iron. Thus, we investigated the biological activity of ABS-derived iron on iron-regulated genes during iron-deficiency anemia (IDA). IDA We selected Caco-2 and HepG2 cell lines as in vitro models of human intestine and liver, respectively. Iron deficiency anemia was induced by deferoxamine. The cells were treated with ferric ammonium citrate (FAC) and ABS. Messenger RNA levels of iron-regulated genes were analyzed by quantitative reverse transcription polymerase chain reaction to elucidate whether iron in ABS behaved similar to inorganic iron (FAC) during IDA. The results showed that ABS-derived iron influenced transcriptions of iron-regulated marker genes, including divalent metal transporter (Dmt1), transferrin receptor (TfR), ankyrin repeat domain 37 (Ankrd37), and hepcidin (Hamp) in IDA-induced Caco-2 and HepG2 cells. Our results suggest that when ABS is used to stop tissue bleeding, it might have an ability to reduce levels of IDA.en_US
dc.language.isoengen_US
dc.publisherSAGE Publicationsen_US
dc.relation.isversionof10.1177/1076029617737838en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAnkaferd Blood Stopperen_US
dc.subjectGene regulationen_US
dc.subjectIron-deficiency anemiaen_US
dc.subjectHepG2 cellsen_US
dc.subjectIronen_US
dc.titleAnkaferd influences mRNA expression of iron-regulated genes during iron-deficiency anemiaen_US
dc.typearticleen_US
dc.contributor.authorIDTR124535en_US
dc.contributor.institutionauthorGüleç, Afife
dc.contributor.institutionauthorGüleç, Şükrü
dc.relation.journalClinical and Applied Thrombosis/Hemostasisen_US
dc.contributor.departmentIzmir Institute of Technology. Food Engineeringen_US
dc.identifier.volume24en_US
dc.identifier.issue6en_US
dc.identifier.startpage960en_US
dc.identifier.endpage964en_US
dc.identifier.wosWOS:000440019200016
dc.identifier.scopusSCOPUS:2-s2.0-85041922240
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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