Show simple item record

dc.contributor.authorAlural, Begüm
dc.contributor.authorDuran, Gizem Ayna
dc.contributor.authorTüfekçi, Kemal Uğur
dc.contributor.authorAllmer, Jens
dc.contributor.authorOnkal, Zeynep
dc.contributor.authorTunalı, Doğa
dc.contributor.authorGenç, Kürşad
dc.contributor.authorGenç, Şermin
dc.date.accessioned2017-05-25T07:43:47Z
dc.date.available2017-05-25T07:43:47Z
dc.date.issued2014-09
dc.identifier.citationAlural, B., Duran, G.A., Tüfekcç, K.U., Allmer, J., Onkal, Z., Tunalı, D., Genç, K., and Genç, Ş. (2014). EPO mediates neurotrophic, neuroprotective, anti-oxidant, and anti-apoptotic effects via downregulation of miR-451 and miR-885-5p in SH-SY5Y neuron-like cells. Frontiers in Immunology, 5(SEP). doi:10.3389/fimmu.2014.00475en_US
dc.identifier.issn1664-3224
dc.identifier.urihttps://doi.org/10.3389/fimmu.2014.00475
dc.identifier.urihttp://hdl.handle.net/11147/5607
dc.description.abstractErythropoietin (EPO) is a neuroprotective cytokine, which has been applied in several animal models presenting neurological disorders. One of the proposed modes of action resulting in neuroprotection is post-transcriptional gene expression regulation. This directly brings to mind microRNAs (miRNAs), which are small non-coding RNAs that regulate gene expression at the post-transcriptional level. It has not yet been evaluated whether miRNAs participate in the biological effects of EPO or whether it, inversely, modulates specific miRNAs in neuronal cells. In this study, we employed miRNA and mRNA arrays to identify how EPO exerts its biological function. Notably, miR-451 and miR-885-5p are downregulated in EPO-treated SH-SY5Y neuronal-like cells. Accordingly, target prediction and transcriptome analysis of cells treated with EPO revealed an alteration of the expression of genes involved in apoptosis, cell survival, proliferation, and migration. Low expression of miRNAs in SH-SY5Y was correlated with high expression of their target genes, vascular endothelial growth factor A, matrix metallo peptidase 9 (MMP9), cyclin-dependent kinase 2 (CDK2), erythropoietin receptor, Mini chromosome maintenance complex 5 (MCM5), B-cell lymphoma 2 (BCL2), and Galanin (GAL). Cell viability, apoptosis, proliferation, and migration assays were carried out for functional analysis after transfection with miRNA mimics, which inhibited some biological actions of EPO such as neuroprotection, anti-oxidation, anti-apoptosis, and migratory effects. In this study, we report for the first time that EPO downregulates the expression of miRNAs (miR-451 and miR-885-5p) in SH-SY5Y neuronal-like cells. The correlation between the over-expression of miRNAs and the decrease in EPO-mediated biological effects suggests that miR-451 and miR-885-5p may play a key role in the mediation of biological function.en_US
dc.description.sponsorshipDokuz Eylul University (2009-KB-SAG-46/2009-KB-SAG-78)en_US
dc.language.isoengen_US
dc.publisherFrontiers Mediaen_US
dc.relation.isversionof10.3389/fimmu.2014.00475en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCell deathen_US
dc.subjectErythropoietinen_US
dc.subjectMigrationen_US
dc.subjectProliferationen_US
dc.subjectMiRNAen_US
dc.subjectNeurite outgrowthen_US
dc.subjectOxidative stressen_US
dc.titleEPO mediates neurotrophic, neuroprotective, anti-oxidant, and anti-apoptotic effects via downregulation of miR-451 and miR-885-5p in SH-SY5Y neuron-like cellsen_US
dc.typearticleen_US
dc.contributor.authorIDTR107974en_US
dc.contributor.institutionauthorAllmer, Jens
dc.relation.journalFrontiers in Immunologyen_US
dc.contributor.departmentİYTE, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.identifier.volume5en_US
dc.identifier.issueSEPen_US
dc.identifier.wosWOS:000354452400001
dc.identifier.scopusSCOPUS:2-s2.0-84918542258
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record