Please use this identifier to cite or link to this item: https://hdl.handle.net/11147/5196
Title: Development of EST-SSR markers for diversity and breeding studies in opium poppy
Authors: Şelale, Hatice
Çelik, İbrahim
Gültekin, Visam
Allmer, Jens
Doğanlar, Sami
Frary, Anne
Keywords: Expressed sequence tag
Genetic diversity
Genic SSRs
Microsatellites
Papaver somniferum L.
Simple sequence repeat
Issue Date: Jun-2013
Publisher: John Wiley and Sons Inc.
Source: Şelale, H., Çelik, I., Gültekin, V., Allmer, J., Doǧanlar, S., and Frary, A. (2013). Development of EST-SSR markers for diversity and breeding studies in opium poppy. Plant Breeding, 132(3), 344-351. doi:10.1111/pbr.12059
Abstract: All publicly available opium poppy expressed sequence tag (EST) sequences, totalling 20 885, were assembled into unigenes and examined for simple sequence repeats (SSRs). Nearly 19% of the 14 957 unigenes contained SSRs with 4% harbouring more than one SSR. Average density of the SSRs was 1 SSR per 3.6 kb of non-redundant EST sequence. Trinucleotide SSRs were most frequently identified (39%), and many of the most prevalent motifs were AT-rich. Flanking primers were designed for 86% of the SSRs and 67 primer pairs were tested on 37 opium poppy accessions and seven related species. All markers were transferable to the related species. Polymorphism information content (PIC) values for the markers were intermediate for comparisons within opium poppy (average of 0.27) and slightly higher for comparisons across species (average of 0.29). The markers were found to be useful for diversity analysis as they successfully distinguished among Turkish opium poppy accessions and land races.
URI: https://doi.org/10.1111/pbr.12059
http://hdl.handle.net/11147/5196
ISSN: 0179-9541
1439-0523
0179-9541
Appears in Collections:Molecular Biology and Genetics / Moleküler Biyoloji ve Genetik
Scopus İndeksli Yayınlar Koleksiyonu / Scopus Indexed Publications Collection
WoS İndeksli Yayınlar Koleksiyonu / WoS Indexed Publications Collection

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