Browsing 1. Fen Fakültesi / Faculty of Science by Department "Izmir Institute of Technology. Molecular Biology and Genetics"
Now showing items 1-20 of 89
-
1.55 Å-resolution structure of ent-copalyl diphosphate synthase and exploration of general acid function by site-directed mutagenesis
(Elsevier, 2014)Background The diterpene cyclase ent-copalyl diphosphate synthase (CPS) catalyzes the first committed step in the biosynthesis of gibberellins. The previously reported 2.25 Å resolution crystal structure of CPS complexed ... -
5-Fluorouracil signaling through a calcium-calmodulin-dependent pathway is required for p53 activation and apoptosis in colon carcinoma cells
(Nature Publishing Group, 2013-09)5-Fluorouracil (5-FU) is an anti-metabolite that is in clinical use for treatment of several cancers. In cells, it is converted into three distinct fluoro-based nucleotide analogs, which interfere with DNA synthesis and ... -
Absence of superoxide dismutase activity causes nuclear DNA fragmentation during the aging process
(Elsevier, 2014-02)Superoxide dismutases (SOD) serve as an important antioxidant defense mechanism in aerobic organisms, and deletion of these genes shortens the replicative life span in the budding yeast Saccharomyces cerevisiae. Even though ... -
Advanced backcross QTL analysis of a Lycopersicon esculentum x L. pennellii cross and identification of possible orthologs in the Solanaceae
(Springer, 2004-02)In this study, the advanced backcross QTL (AB-QTL) mapping strategy was used to identify loci for yield, processing and fruit quality traits in a population derived from the interspecific cross Lycopersicon esculentum E6203 ... -
Aggregatibacter actinomycetemcomitans GroEL Protein Promotes Conversion of Human CD4+ T Cells into IFNγ IL10 Producing Tbet+ Th1 Cells
(Public Library of Science, 2012-11)One of the heat shock family protein (Hsp) expressing bacteria is the gram negative, periodontal pathogen Aggregatibacter actinomycetemcomitans (Aa). A. actinomycetemcomitans' Hsp is a 64-kDa GroEL-protein, which has been ... -
The aggregatibacter actinomycetemcomitans heat shock protein GroEL interacts directly with human peripheral blood T cells
(TÜBİTAK, 2016)Heat shock family protein GroEL of Aggregatibacter actinomycetemcomitans (Aa) has antigenic properties. We previously demonstrated that A. actinomycetemcomitans GroEL-like protein affects human CD4 T cells by converting ... -
Agrobacterium rhizogenes-mediated transformation and its biotechnological applications in crops
(Springer, 2013)The history of Agrobacterium-related plant biotechnology goes back for more than three decades with the discovery of molecular mechanisms of crown gall disease in plants. After 1980s, gene technologies began developing ... -
Analysis of EST-SSRs in silver birch (Betula pendula Roth.)
(Northeast Forestry University, 2016-06)Simple sequence repeats (SSRs) defined as sequence repeat units between 1 and 6 bp occur abundantly in both coding and non-coding regions in eukaryotic genomes and these repeats can affect gene expression. In this study, ... -
Antifungal activity of biopolymers containing natamycin and rosemary extract against Aspergillus niger and Penicillium roquefortii
(Wiley-Blackwell, 2008-11)Antimicrobial agent-releasing films have been proposed as an effective way of inhibiting chiefly surface spoilage of food products. Antifungal activities of natamycin (NA), rosemary extract (RE) and NA + RE were tested ... -
Antimicrobial and antioxidant activity of edible zein films incorporated with lysozyme, albumin proteins and disodium EDTA
(Elsevier, 2007-01)In this study, partially purified lysozyme was incorporated into zein films in combination with chickpea albumin extract (CPAE), bovine serum albumin (BSA) and disodium EDTA. The zein films showed an inherent free radical ... -
Autologous rabbit adipose tissue-derived mesenchymal stromal cells for the treatment of bone injuries with distraction osteogenesis
(Elsevier, 2013-06)Background aims: Adipose tissue-derived mesenchymal stromal cells (MSCs) have a higher capacity for proliferation and differentiation compared with other cell lineages. Although distraction osteogenesis is the most important ... -
Biofabrication of in situ self assembled 3D cell cultures in a weightlessness environment generated using magnetic levitation
(Nature Publishing, 2018-12)Magnetic levitation though negative magnetophoresis is a novel technology to simulate weightlessness and has recently found applications in material and biological sciences. Yet little is known about the ability of the ... -
Biofilm formation by Staphylococcus epidermidis on nitrogen ion implanted CoCrMo alloy material
(Wiley, 2007-06)Staphylococcus epidermidis is the primary cause of medical device-related infections due to its adhesion and biofilm forming abilities on biomaterial surfaces. For this reason development of new materials and surfaces to ... -
Biotechnology for enhanced nutritional quality in plants
(Taylor & Francis, 2013-09)With almost 870 million people estimated to suffer from chronic hunger worldwide, undernourishment represents a major problem that severely affects people in developing countries. In addition to undernourishment, micronutrient ... -
Bisphosphonate treatment and radiotherapy in metastatic breast cancer
(Humana Press, 2008-09)Patients with advanced breast cancer frequently develop metastasis to bone. Bone metastasis results in intractable pain and high risk of pathologic fractures due to osteolysis. The treatment of breast cancer patients with ... -
Can MiRBase provide positive data for machine learning for the detection of MiRNA hairpins?
(Informationsmanagement in der Biotechnologie e.V., 2013)Experimental detection and validation of miRNAs is a tedious, time-consuming, and expensive process. Computational methods for miRNA gene detection are being developed so that the number of candidates that need experimental ... -
Cell proliferation and cytotoxicity assays
(Bentham Science, 2016-11)Cell viability is defined as the number of healthy cells in a sample and proliferation of cells is a vital indicator for understanding the mechanisms in action of certain genes, proteins and pathways involved cell survival ... -
Characterization of two genes encoding metal tolerance proteins from Beta vulgaris subspecies maritima that confers manganese tolerance in yeast
(Springer, 2013)Manganese (Mn2+) is an essential micronutrient in plants. However increased Mn2+ levels are toxic to plant cells. Metal tolerance proteins (MTPs), member of cation diffusion facilitator protein (CDF) family, have important ... -
Checkpoint deficient rad53-11 yeast cannot accumulate dNTPs in response to DNA damage
(Elsevier, 2007-02)Deoxyribonucleotide pools are maintained at levels that support efficient and yet accurate DNA replication and repair. Rad53 is part of a protein kinase regulatory cascade that, conceptually, promotes dNTP accumulation in ... -
Comparative fine mapping of fruit quality QTLs on chromosome 4 introgressions derived from two wild tomato species
(Kluwer Academic Publishers, 2004)Despite their unsuitability for agricultural production, the wild relatives of crop species represent a largely untapped resource of novel QTLs potentially useful for crop plant improvement. In this regard, previous ...
