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dc.contributor.authorBaran, Yusuf
dc.contributor.authorUral, Ali Uğur
dc.contributor.authorAvcu, Ferit
dc.contributor.authorSarper, Meral
dc.contributor.authorElçi, Pınar
dc.contributor.authorPekel, Aysel
dc.date.accessioned2016-10-20T12:14:28Z
dc.date.available2016-10-20T12:14:28Z
dc.date.issued2008
dc.identifier.citationBaran, Y., Ural, A. U., Avcu, F., Sarper, M., Elçi, P., and Pekel, A. (2008). Optimization of transfection of green fluorescent protein in pursuing mesenchymal stem cells in vivo. Turkish Journal of Hematology, 25(4), 172-175.en_US
dc.identifier.issn1300-7777
dc.identifier.urihttp://hdl.handle.net/11147/2294
dc.description.abstractObjective: Green Fluorescent Protein (GFP) has been used as a marker of gene expression and a single cell marker in living organisms in cell biology studies. The important areas that GFP is used are expression levels of different genes in different organisms by inserting GFP in these genes and as a marker in living cells. In this study, we tried to optimize transfection of mesenchymal stem cells, (MSCs) used for regeneration of damaged tissues in animals, by GFP containing plasmid vector by which MSCs can be followed in vivo. Material and Methods: To this aim, phM-GFP plasmid vector carrying GFP gene and effectene transfection reagent were used. Result: The data revealed that twice transfection of MSCs resulted in higher expression of GFP for longer times as compared to once transfected MSCs. On the other hand, leaving the chemical transfection agents in the medium induced apoptosis after a while. Conclusion: As a conclusion we suggest the transfection of MSCs twice with 48 hours interval and removal of transfection agents after 8 hours which removed toxic and apoptotic effects of the chemicals.en_US
dc.language.isoengen_US
dc.publisherTurkish Society of Hematologyen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectEffecteneen_US
dc.subjectGreen fluorescent proteinen_US
dc.subjectMesenchymal stem cellsen_US
dc.subjectTransfectionen_US
dc.titleOptimization of transfection of green fluorescent protein in pursuing mesenchymal stem cells in vivoen_US
dc.title.alternativeMezankimal kök hücrelerin in vivo takibinde yeşil floresan protein aktarılmasının optimizasyonuen_US
dc.typearticleen_US
dc.contributor.authorIDTR119193en_US
dc.contributor.institutionauthorBaran, Yusuf
dc.relation.journalTurkish Journal of Hematologyen_US
dc.contributor.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume25en_US
dc.identifier.issue4en_US
dc.identifier.startpage172en_US
dc.identifier.endpage175en_US
dc.identifier.scopusSCOPUS:2-s2.0-84860397246
dc.relation.publicationcategoryMakale - Ulusal Hakemli Dergi - Kurum Öğretim Elemanıen_US


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