Show simple item record

dc.contributor.authorMuid, Khandaker Ashfaqul
dc.contributor.authorKarakaya, Hüseyin Çaǧlar
dc.contributor.authorKoç, Ahmet
dc.date.accessioned2017-05-17T12:38:07Z
dc.date.available2017-05-17T12:38:07Z
dc.date.issued2014-02
dc.identifier.citationMuid, K.A., Karakaya, H.Ç., and Koç, A. (2014). Absence of superoxide dismutase activity causes nuclear DNA fragmentation during the aging process. Biochemical and Biophysical Research Communications, 444(2), 260-263. doi:10.1016/j.bbrc.2014.01.056en_US
dc.identifier.issn0006-291X
dc.identifier.urihttps://doi.org/10.1016/j.bbrc.2014.01.056
dc.identifier.urihttp://hdl.handle.net/11147/5542
dc.description.abstractSuperoxide dismutases (SOD) serve as an important antioxidant defense mechanism in aerobic organisms, and deletion of these genes shortens the replicative life span in the budding yeast Saccharomyces cerevisiae. Even though involvement of superoxide dismutase enzymes in ROS scavenging and the aging process has been studied extensively in different organisms, analyses of DNA damages has not been performed for replicatively old superoxide dismutase deficient cells. In this study, we investigated the roles of SOD1, SOD2 and CCS1 genes in preserving genomic integrity in replicatively old yeast cells using the single cell comet assay. We observed that extend of DNA damage was not significantly different among the young cells of wild type, sod1Δ and sod2Δ strains. However, ccs1Δ mutants showed a 60% higher amount of DNA damage in the young stage compared to that of the wild type cells. The aging process increased the DNA damage rates 3-fold in the wild type and more than 5-fold in sod1Δ, sod2Δ, and ccs1Δ mutant cells. Furthermore, ROS levels of these strains showed a similar pattern to their DNA damage contents. Thus, our results confirm that cells accumulate DNA damages during the aging process and reveal that superoxide dismutase enzymes play a substantial role in preserving the genomic integrity in this process.en_US
dc.description.sponsorshipState Planning Organization of Turkey (2008K120730); Scientific and Technological Research Council of Turkey (TUBITAK-BIDEP)en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/j.bbrc.2014.01.056en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAgingen_US
dc.subjectAntioxidanten_US
dc.subjectComet assayen_US
dc.subjectDNA damageen_US
dc.subjectOxidative stressen_US
dc.subjectReactive oxygen speciesen_US
dc.titleAbsence of superoxide dismutase activity causes nuclear DNA fragmentation during the aging processen_US
dc.typearticleen_US
dc.contributor.authorIDTR24898en_US
dc.contributor.iztechauthorMuid, Khandaker Ashfaqul
dc.contributor.iztechauthorKarakaya, Hüseyin Çaǧlar
dc.contributor.iztechauthorKoç, Ahmet
dc.relation.journalBiochemical and Biophysical Research Communicationsen_US
dc.contributor.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume444en_US
dc.identifier.issue2en_US
dc.identifier.startpage260en_US
dc.identifier.endpage263en_US
dc.identifier.wosWOS:000331923500026
dc.identifier.scopusSCOPUS:2-s2.0-84894544368
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record