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dc.contributor.authorAtik, Ahmet Emin
dc.contributor.authorYalçın, Talat
dc.date.accessioned2016-12-23T08:43:32Z
dc.date.available2016-12-23T08:43:32Z
dc.date.issued2011-01-27
dc.identifier.citationAtik, A. E., and Yalçın, T. (2011). A Systematic study of acidic peptides for b-type sequence scrambling. Journal of The American Society for Mass Spectrometry, 22(1), 38-48. doi:10.1007/s13361-010-0018-3en_US
dc.identifier.issn1879-1123
dc.identifier.urihttp://doi.org/10.1007/s13361-010-0018-3
dc.identifier.urihttp://hdl.handle.net/11147/2662
dc.description.abstractA systematic study was carried out to examine the effects of acidic amino acid residues and the position of the acidic group on the cyclization of b ions. The study utilized the model C-terminal amidated eptides AAAAAA, AXAAAAA, AAXAAAA, AAAXAAA, AAAAXAA, AAAAAXA, AAAAAAX, XXAAAAAA, AAXXAAAA, AAAAXXAA, and AAAAAAXX, where X is a glutamic acid (E) or aspartic acid (D) residue. The CID mass spectra of bn (where n=7 and 8) ions derived from XAAAAAA, AAAXAAA, AAAAAAX and XXAAAAAA, AAXXAAAA, AAAAXXAA, and AAAAAAXX exhibited very similar fragmentation patterns for both the glutamic and the aspartic acid peptide series. The CID mass spectra of MH+ derived from model peptides presented substantial direct and non-direct sequence bions. The results indicate that b ions produced from acidic peptides can also undergo head-to-tail cyclization, which is the reason for the formation of the non-direct sequence b ions. The bion spectra derived from the peptides became more complex as the number of acidic residues in the peptides increased. Side chains of glutamic and aspartic acid did not inhibit the cyclization of the b ions. Substantial water elimination was observed in all CID spectra of b7 and b8 ions. Finally, the preferential cleavage of glutamic or aspartic acid residues from macrocyclic structures of b ions was also investigated under various collision energy conditions.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey, TUBITAKen_US
dc.language.isoengen_US
dc.publisherAmerican Society for Mass Spectrometryen_US
dc.relation.isversionof10.1007/s13361-010-0018-3en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectb-type sequence scramblingen_US
dc.subjectMacrocyclizationen_US
dc.subjectGlutamic aciden_US
dc.subjectAspartic aciden_US
dc.subjectAdjacent acidic residuesen_US
dc.titleA Systematic study of acidic peptides for b-type sequence scramblingen_US
dc.typearticleen_US
dc.contributor.authorIDTR130617en_US
dc.contributor.iztechauthorAtik, Ahmet Emin
dc.contributor.iztechauthorYalçın, Talat
dc.relation.journalJournal of The American Society for Mass Spectrometryen_US
dc.contributor.departmentİYTE, Fen Fakültesi, Kimya Bölümüen_US
dc.identifier.volume22en_US
dc.identifier.issue1en_US
dc.identifier.startpage38en_US
dc.identifier.endpage48en_US
dc.identifier.wosWOS:000287696300005
dc.identifier.scopusSCOPUS:2-s2.0-79952514268
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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