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dc.contributor.authorÇınar, Çelenk
dc.contributor.authorApaydın, Özgür
dc.contributor.authorYenidünya, Ali Fazıl
dc.contributor.authorHarsa, Hayriye Şebnem
dc.contributor.authorGüneş, Hatice
dc.date.accessioned2016-11-11T08:01:42Z
dc.date.available2016-11-11T08:01:42Z
dc.date.issued2008-02
dc.identifier.citationÇınar, Ç., Apaydın, Ö., Yenidünya, A. F., Harsa, H. Ş., and Güneş, H. (2008). Isolation and characterization of Bacillus thuringiensis strains from olive-related habitats in Turkey. Journal of Applied Microbiology, 104(2), 515-525. doi:10.1111/j.1365-2672.2007.03571.xen_US
dc.identifier.issn1364-5072
dc.identifier.urihttp://doi.org/10.1111/j.1365-2672.2007.03571.x
dc.identifier.urihttp://hdl.handle.net/11147/2423
dc.description.abstractAims: To isolate Bacillus thuringiensis strains from different olive-related habitats (olive groves and olive oil factories) in Turkey and to characterize these strains by molecular methods. Methods and Results: A total of 150 samples, consisting of olive grove soil, green olive leaves, olive leaf residues, animal faeces, olive pomace and dust, were examined for the presence of B. thuringiensis. One hundred B. thuringiensis strains were isolated from 54 environmental samples (36%) and characterized in terms of crystal morphology, cry and cyt gene content by polymerase chain reaction, plasmid profiles and 16S-internal transcribed spacer ribosomal DNA restriction fragment length polymorphism (16S-ITS rDNA RFLP). The highest percentage of samples containing B. thuringiensis was found in 38 out of 54 total soil samples (70%). Of the 100 B. thuringiensis isolates, the most frequent crystal shapes were irregularly shaped (24%), spherical-irregular pointed (19%), cuboidal (17%) and spherical (16%). The cry1 plus cry4 genotype was the most abundant genotype in our collection (21%). RFLP analysis of the amplified 16S-ITS rDNA revealed 11 distinct patterns for the isolates and 10 reference strains. Conclusions: Bacillus thuringiensis isolates showed a great genetic diversity and crystal shape heterogeneity. Significance and Impact of the Study: This is the first study on the isolation and characterization of B. thuringiensis from olive-related habitats in Turkey. No correlation was observed between the cry genotypes and insecticidal crystal shapes of the isolates. Restriction profiles of 23% of the isolates were found to be different from those of the 10 reference strains used.en_US
dc.description.sponsorshipState Planning Organization, DPT (2002K-120390)en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1111/j.1365-2672.2007.03571.xen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBacillus thuringiensisen_US
dc.subjectCharacterizationen_US
dc.subjectCrystal proteinen_US
dc.subjectPolymerase chain reactionen_US
dc.titleIsolation and characterization of Bacillus thuringiensis strains from olive-related habitats in Turkeyen_US
dc.typearticleen_US
dc.contributor.authorIDTR9626en_US
dc.contributor.iztechauthorÇınar, Çelenk
dc.contributor.iztechauthorApaydın, Özgür
dc.contributor.iztechauthorHarsa, Hayriye Şebnem
dc.relation.journalJournal of Applied Microbiologyen_US
dc.contributor.departmentIzmir Institute of Technology. Food Engineeringen_US
dc.identifier.volume104en_US
dc.identifier.issue2en_US
dc.identifier.startpage515en_US
dc.identifier.endpage525en_US
dc.identifier.wosWOS:000252496100020
dc.identifier.scopusSCOPUS:2-s2.0-38349179668
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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