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dc.contributor.authorApaydın, Özgür
dc.contributor.authorYenidünya, Ali Fazıl
dc.contributor.authorHarsa, Hayriye Şebnem
dc.contributor.authorGüneş, Hatice
dc.date.accessioned2016-07-22T11:18:36Z
dc.date.available2016-07-22T11:18:36Z
dc.date.issued2005-04
dc.identifier.citationApaydın, Ö., Yenidünya, A.F., Harsa, H.Ş., and Güneş, H. (2005). Isolation and characterization of Bacillus thuringiensis strains from different grain habitats in Turkey. World Journal of Microbiology and Biotechnology, 21(3), 285-292. doi:10.1007/s11274-004-3633-yen_US
dc.identifier.issn0959-3993
dc.identifier.urihttps://doi.org/10.1007/s11274-004-3633-y
dc.identifier.urihttp://hdl.handle.net/11147/1971
dc.description.abstractBacillus thuringiensis (Bt) is a gram-positive, spore-forming bacterium and it produces insecticidal crystal (cry) proteins during sporulation. Because the genetic diversity and toxic potential of Bt strains differ from region to region, strains have been collected and characterized all over the world. The aim of this study is to isolate Bt strains in grain-related habitats in Turkey and to characterize them on the basis of crystal morphology, cry gene content, and chromosomal and plasmid DNA profiles. Four approaches were taken analysis with phase contrast (PC) microscopy, polymerase chain reaction (PCR), pulsed field gel electrophoresis (PFGE) and plasmid isolation. Ninety-six samples were collected from Central Anatolia and the Aegean region. Bt was isolated from 61 of 96 samples (63.5) and 500 Bt-like colonies were obtained. One hundred and sixty three of the colonies were identified as Bt based on cry protein formation using PC microscopy. Among the examined colonies, the overall proportion identified (as Bt index) was 0.33. We found that 103 isolates were positive for the five different cry genes (cry1, cry2, cry3, cry4 and cry9) examined with PCR. In addition, plasmid profiling of 37 cry gene-positive isolates indicated that the 15 kb plasmid band was present in all isolates; however, 11 of 37 isolates had more than one plasmid band at different sizes. Finally, chromosomal DNA profiling by PFGE gave rise to different DNA patterns for isolates containing the same cry gene which suggests a high level of diversity among the Bt strains isolated.en_US
dc.description.sponsorshipİYTE: 2002 IYTE43; DPT: 2002K-1207390en_US
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.relation.isversionof10.1007/s11274-004-3633-yen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBacillus thuringiensisen_US
dc.subjectCry genesen_US
dc.subjectGrain habitatsen_US
dc.subjectIsolationen_US
dc.subjectPFGE profilesen_US
dc.subjectPlasmiden_US
dc.subjectBacteriaen_US
dc.titleIsolation and characterization of Bacillus thuringiensis strains from different grain habitats in Turkeyen_US
dc.typearticleen_US
dc.contributor.authorIDTR42080en_US
dc.contributor.authorIDTR9626en_US
dc.contributor.authorIDTR2082en_US
dc.contributor.iztechauthorApaydın, Özgür
dc.contributor.iztechauthorYenidünya, Ali Fazıl
dc.contributor.iztechauthorHarsa, Hayriye Şebnem
dc.contributor.iztechauthorGüneş, Hatice
dc.relation.journalWorld Journal of Microbiology and Biotechnologyen_US
dc.contributor.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.contributor.departmentIzmir Institute of Technology. Food Engineeringen_US
dc.identifier.volume21en_US
dc.identifier.issue3en_US
dc.identifier.startpage285en_US
dc.identifier.endpage292en_US
dc.identifier.wosWOS:000230567100012
dc.identifier.scopusSCOPUS:2-s2.0-23744477140
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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