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dc.contributor.authorAcar, İlhan Erkin
dc.contributor.authorSaçar Demirci, Müşerref Duygu
dc.contributor.authorGroß, Uwe
dc.contributor.authorAllmer, Jens
dc.date.accessioned2019-02-04T11:15:14Z
dc.date.available2019-02-04T11:15:14Z
dc.date.issued2018-01
dc.identifier.citationAcar, İ. E., Saçar Demirci, M. D., Groß, U., and Allmer, J. (2018). The expressed MicroRNA-mRNA interactions of Toxoplasma gondii. Frontiers in Microbiology, 8(JAN). doi:10.3389/fmicb.2017.02630en_US
dc.identifier.issn1664-302X
dc.identifier.urihttps://doi.org/10.3389/fmicb.2017.02630
dc.identifier.urihttp://hdl.handle.net/11147/7082
dc.description.abstractMicroRNAs (miRNAs) are involved in post-transcriptional modulation of gene expression and thereby have a large influence on the resulting phenotype. We have previously shown that miRNAs may be involved in the communication between Toxoplasma gondii and its hosts and further confirmed a number of proposed specific miRNAs. Yet, little is known about the internal regulation via miRNAs in T. gondii. Therefore, we predicted pre-miRNAs directly from the type II ME49 genome and filtered them. For the confident hairpins, we predicted the location of the mature miRNAs and established their target genes. To add further confidence, we evaluated whether the hairpins and their targets were co-expressed. Such co-expressed miRNA and target pairs define a functional interaction. We extracted all such functional interactions and analyzed their differential expression among strains of all three clonal lineages (RH, PLK, and CTG) and between the two stages present in the intermediate host (tachyzoites and bradyzoites). Overall, we found ~65,000 expressed interactions of which ~5,500 are differentially expressed among strains but none are significantly differentially expressed between developmental stages. Since miRNAs and target decoys can be used as therapeutics we believe that the list of interactions we provide will lead to novel approaches in the treatment of toxoplasmosis.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (113E326), Amazon Web Servicesen_US
dc.language.isoengen_US
dc.publisherFrontiers Media S.A.en_US
dc.relationinfo:eu-repo/grantAgreement/TUBITAK/EEEAG/113E326en_US
dc.relation.isversionof10.3389/fmicb.2017.02630en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDifferential expressionen_US
dc.subjectExpression analysisen_US
dc.subjectMicroRNAsen_US
dc.subjectMiRNA targeten_US
dc.subjectToxoplasma gondiien_US
dc.titleThe expressed MicroRNA-mRNA interactions of Toxoplasma gondiien_US
dc.typearticleen_US
dc.contributor.authorIDTR114170en_US
dc.contributor.authorIDTR107974en_US
dc.contributor.iztechauthorAcar, İlhan Erkin
dc.contributor.iztechauthorSaçar Demirci, Müşerref Duygu
dc.contributor.iztechauthorAllmer, Jens
dc.relation.journalFrontiers in Microbiologyen_US
dc.contributor.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume8en_US
dc.identifier.issueJANen_US
dc.identifier.wosWOS:000419343800004
dc.identifier.scopusSCOPUS:2-s2.0-85040027421
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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