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dc.contributor.authorUz, Metin
dc.contributor.authorAlsoy Altınkaya, Sacide
dc.contributor.authorMallapragada, Surya K.
dc.date.accessioned2017-11-23T08:31:24Z
dc.date.available2017-11-23T08:31:24Z
dc.date.issued2017-08
dc.identifier.citationUz, M., Alsoy Altınkaya, S., and Mallapragada, S. K. (2017). Stimuli responsive polymer-based strategies for polynucleotide delivery. Journal of Materials Research, 32(15), 2930-3953. doi:10.1557/jmr.2017.116en_US
dc.identifier.issn0884-2914
dc.identifier.urihttp://doi.org/10.1557/jmr.2017.116
dc.identifier.urihttp://hdl.handle.net/11147/6491
dc.description.abstractIn recent years, stimuli responsive polymer based gene delivery vehicle design for cancer treatment and treatment of other genetic disorders has received extensive attention. Early studies focusing on DNA delivery have been facilitated by functional polymers and this area has seen further growth spurred by recent gene silencing strategies developed for small RNA [i.e., small interfering RNA (siRNA) or micro RNA (miRNA)] delivery. DNA and small RNAs possess analogous properties; however, their explicit differences define the specific challenges associated with the delivery route and the design of functional materials to overcome distinct challenges. Apart from classical gene delivery, the recent advances in genome editing have revealed the necessity of new delivery devices for genome editing tools. A system involving CRISPR (clustered, regularly interspaced, short palindromic repeats) and an endonuclease CRISPR-associated protein 9 (Cas9) coupled with a short, single-guide RNA (sgRNA) has emerged as a promising tool for genome editing along with functional delivery systems. For all these nucleic acid based treatments, the internal or external physiochemical changes in the biological tissue/cells play a major role in the design of stimuli responsive delivery materials for both in vitro and in vivo applications. This review emphasizes the recent advances in the use of pH, temperature, and redox potential-responsive polymers overcoming hurdles for delivery of gene and gene editing tools for both in vitro and in vivo applications. Specifically the chapter focuses on recently proposed delivery strategies, types of delivery systems, and polymer synthesis/modification methods. The recent advances in CRISPR/Cas9-sgRNA technology and delivery are also described in a separate section. The review ends with current clinical trials, concluding remarks, and future perspectives.en_US
dc.description.sponsorshipUS Army Medical Research and Materiel Command (W81XWH-10-1-0806); Stanley Endowed Chair in Interdisciplinary Engineeringen_US
dc.language.isoengen_US
dc.publisherCambridge University Pressen_US
dc.relation.isversionof10.1557/jmr.2017.116en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectFunctional polymersen_US
dc.subjectStimuli responsive polymersen_US
dc.subjectGene deliveryen_US
dc.subjectCRISPR/Cas9en_US
dc.subjectSmall interfering RNAen_US
dc.titleStimuli responsive polymer-based strategies for polynucleotide deliveryen_US
dc.typereviewen_US
dc.contributor.authorIDTR2091en_US
dc.contributor.iztechauthorAlsoy Altınkaya, Sacide
dc.relation.journalJournal of Materials Researchen_US
dc.contributor.departmentİYTE, Mühendislik Fakültesi, Kimya Mühendisliği Bölümüen_US
dc.identifier.volume32en_US
dc.identifier.issue15en_US
dc.identifier.startpage2930en_US
dc.identifier.endpage2953en_US
dc.identifier.wosWOS:000407745200007
dc.identifier.scopusSCOPUS:2-s2.0-85018846926
dc.relation.publicationcategoryDiğeren_US


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