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dc.contributor.authorRajwar, Deepa
dc.contributor.authorAmmanath, Gopal
dc.contributor.authorCheema, Jamal Ahmed
dc.contributor.authorPalaniappan, Alagappan
dc.contributor.authorYıldız, Ümit Hakan
dc.contributor.authorLiedberg, Bo
dc.date.accessioned2017-08-14T13:06:21Z
dc.date.available2017-08-14T13:06:21Z
dc.date.issued2016-04-20
dc.identifier.citationRajwar, D., Ammanath, G., Cheema, J. A., Palaniappan, A., Yıldız, Ü. H., and Liedberg, B. (2016). Tailoring conformation-induced chromism of polythiophene copolymers for nucleic acid assay at resource limited settings. ACS Applied Materials and Interfaces, 8(13), 8349-8357. doi:10.1021/acsami.5b12171en_US
dc.identifier.issn1944-8244
dc.identifier.urihttp://doi.org/10.1021/acsami.5b12171
dc.identifier.urihttp://hdl.handle.net/11147/6103
dc.description.abstractHere we report on the design and synthesis of cationic water-soluble thiophene copolymers as reporters for colorimetric detection of microRNA (miRNA) in human plasma. Poly(3-alkoxythiophene) (PT) polyelectrolytes with controlled ratios of pendant groups such as triethylamine/1-methyl imidazole were synthesized for optimizing interaction with target miRNA sequence (Tseq). Incorporation of specific peptide nucleic acid (PNA) sequences with the cationic polythiophenes yielded distinguishable responses upon formation of fluorescent PT-PNA-Tseq triplex and weakly fluorescent PT-Tseq duplex, thereby enabling selective detection of target miRNA. Unlike homopolymers of PT (hPT), experimental results indicate the possibility of utilizing copolymers of PT (cPT) with appropriate ratios of pendant groups for miRNA assay in complex matrices such as plasma. As an illustration, colorimetric responses were obtained for lung cancer associated miRNA sequence (mir21) in human plasma, with a detection limit of 10 nM, illustrating the feasibility of proposed methodology for clinical applications without involving sophisticated instrumentation. The described methodology therefore possesses high potential for low-cost nucleic acid assays in resource-limited settings.en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.relation.isversionof10.1021/acsami.5b12171en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectmiRNAen_US
dc.subjectPNAen_US
dc.subjectColorimetric assayen_US
dc.subjectConjugated polyelectrolytesen_US
dc.subjectPolythiopheneen_US
dc.titleTailoring conformation-induced chromism of polythiophene copolymers for nucleic acid assay at resource limited settingsen_US
dc.typearticleen_US
dc.contributor.authorIDTR147447en_US
dc.contributor.iztechauthorYıldız, Ümit Hakan
dc.relation.journalACS Applied Materials and Interfacesen_US
dc.contributor.departmentİYTE, Fen Fakültesi, Kimya Bölümüen_US
dc.identifier.volume8en_US
dc.identifier.issue13en_US
dc.identifier.startpage8349en_US
dc.identifier.endpage8357en_US
dc.identifier.wosWOS:000373748200005
dc.identifier.scopusSCOPUS:2-s2.0-84964617611
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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