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dc.contributor.authorMata-Gomez, Marco A.
dc.contributor.authorYaman, Sena
dc.contributor.authorValencia-Gallegos, Jesus A.
dc.contributor.authorTarı, Canan
dc.contributor.authorRito-Palomares, Marco
dc.contributor.authorGonzalez-Valdez, Jose
dc.date.accessioned2017-08-14T11:57:30Z
dc.date.available2017-08-14T11:57:30Z
dc.date.issued2016-04-22
dc.identifier.citationMata-Gomez, M. A., Yaman, S., Valencia-Gallegos, J. A.,Tarı, C., Rito-Palomares, M. A., and Gonzalez-Valdez, J. (2016). Synthesis of adsorbents with dendronic structures for protein hydrophobic interaction chromatography. Journal of Chromatography A, 1443, 191-200. doi:10.1016/j.chroma.2016.03.057en_US
dc.identifier.issn0021-9673
dc.identifier.urihttp://doi.org/10.1016/j.chroma.2016.03.057
dc.identifier.urihttp://hdl.handle.net/11147/6101
dc.description.abstractHere, we introduced a new technology based on the incorporation of dendrons-branched chemical structures-onto supports for synthesis of HIC adsorbents. In doing so we studied the synthesis and performance of these novel HIC dendron-based adsorbents. The adsorbents were synthesized in a facile two-step reaction. First, Sepharose 4FF (R) was chemically modified with polyester dendrons of different branching degrees i.e. third (G3) or fifth (G5) generations. Then, butyl-end valeric acid ligands were coupled to dendrons via ester bond formation. UV-vis spectrophotometry and FTIR analyses of the modified resins confirmed the presence of the dendrons and their ligands on them. Inclusion of dendrons allowed the increment of ligand density, 82.5 ± 11 and 175.6 ± 5.7 μmol ligand/mL resin for RG3 and RG5, respectively. Static adsorption capacity of modified resins was found to be ~60 mg BSA/mL resin. Interestingly, dynamic binding capacity was higher at high flow rates, 62.5 ± 0.8 and 58.0 ± 0.5 mg/mL for RG3 and RG5, respectively. RG3 was able to separate lipase, β-lactoglobulin and α-chymotrypsin selectively as well as fractionating of a whole proteome from yeast. This innovative technology will improve the existing HIC resin synthesis methods. It will also allow the reduction of the amount of adsorbent used in a chromatographic procedure and thus permit the use of smaller columns resulting in faster processes. Furthermore, this method could potentially be considered as a green technology since both, dendrons and ligands, are formed by ester bonds that are more biodegradable allowing the disposal of used resin waste in a more ecofriendly manner when compared to other exiting resins.en_US
dc.description.sponsorshipBiotechnology and Synthetic Biology Focus Group (002EICIP01--0020DII001); Chemistry and Nanotechnology Department (0020CDB088); National Council on Science and Technology of Mexico (CONACyT) (242286--204152); EU-FP7-Marie Curie Action (PIRSES-GA 2010-269211)en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/j.chroma.2016.03.057en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAdsorbenten_US
dc.subjectDendronsen_US
dc.subjectHydrophobic interaction chromatographyen_US
dc.subjectResinen_US
dc.subjectHydrophobicityen_US
dc.titleSynthesis of adsorbents with dendronic structures for protein hydrophobic interaction chromatographyen_US
dc.typearticleen_US
dc.contributor.authorIDTR12028en_US
dc.contributor.iztechauthorYaman, Sena
dc.contributor.iztechauthorTarı, Canan
dc.relation.journalJournal of Chromatography Aen_US
dc.contributor.departmentİYTE, Mühendislik Fakültesi, Gıda Mühendisliği Bölümüen_US
dc.identifier.volume1443en_US
dc.identifier.startpage191en_US
dc.identifier.endpage200en_US
dc.identifier.wosWOS:000374362600023
dc.identifier.scopusSCOPUS:2-s2.0-84963612245
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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