Show simple item record

dc.contributor.authorBor, Gizem
dc.contributor.authorMytych, Jennifer
dc.contributor.authorZebrowski, Jacek
dc.contributor.authorWnuk, Maciej
dc.contributor.authorŞanlı Mohamed, Gülşah
dc.date.accessioned2017-07-21T10:53:37Z
dc.date.available2017-07-21T10:53:37Z
dc.date.issued2016-11-20
dc.identifier.citationBor, G., Mytych, J., Zebrowski, J., Wnuk, M., and Şanlı Mohamed, G. (2016). Cytotoxic and cytostatic side effects of chitosan nanoparticles as a non-viral gene carrier. International Journal of Pharmaceutics, 513(1-2), 431-437. doi:10.1016/j.ijpharm.2016.09.058en_US
dc.identifier.issn0378-5173
dc.identifier.urihttp://doi.org/10.1016/j.ijpharm.2016.09.058
dc.identifier.urihttp://hdl.handle.net/11147/5987
dc.description.abstractAlthough chitosan nanoparticles (CNs) became a promising tool for several biological and medical applications owing to their inherent biocompatibility and biodegradability features, studies regarding their effects on cytotoxic and cytostatic properties still remain insufficient. Therefore, in the present study, we decided to perform comprehensive analysis of the interactions between CNs–pKindling-Red-Mito (pDNA) and different cell line models derived from blood system and human solid tissues cancers. The resulting CNs-pDNA was investigated in terms of their cellular uptake, transfection efficiency, and physico-chemical, cytotoxic and cytostatic properties. The nanoparticles showed high encapsulation efficiency and physical stability for various formulations even after two days time period. Moreover, high gene expression levels were observed after 96 h of transfection. CNs-pDNA treatment, despite the absence of oxidative stress induction, caused cell cycle arrest in G0/G1 phase and as a consequence led to premature senescence which turned out to be both p21-dependent and p21-independent. Also, observed DNMT2 upregulation may suggest the activation of different pathways protecting from the results of CNs-mediated stress. In conclusion, treatment of different cell lines with CNs-pDNA showed that their biocompatibility was limited and the effects were cell type-dependent.en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/j.ijpharm.2016.09.058en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCancer cellsen_US
dc.subjectChitosanen_US
dc.subjectCytotoxicityen_US
dc.subjectGene deliveryen_US
dc.subjectMonocytesen_US
dc.subjectNanoparticlesen_US
dc.subjectPlasmid DNAen_US
dc.titleCytotoxic and cytostatic side effects of chitosan nanoparticles as a non-viral gene carrieren_US
dc.typearticleen_US
dc.contributor.authorIDTR115002en_US
dc.contributor.iztechauthorBor, Gizem
dc.contributor.iztechauthorŞanlı Mohamed, Gülşah
dc.relation.journalInternational Journal of Pharmaceuticsen_US
dc.contributor.departmentİYTE, Fen Fakültesi, Kimya Bölümüen_US
dc.identifier.volume513en_US
dc.identifier.issue1-2en_US
dc.identifier.startpage431en_US
dc.identifier.endpage437en_US
dc.identifier.wosWOS:000386779700044
dc.identifier.scopusSCOPUS:2-s2.0-84988596753
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record