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dc.contributor.authorHorzum, Utku
dc.contributor.authorÖzdil, Berrin
dc.contributor.authorPesen Okvur, Devrim
dc.date.accessioned2017-07-06T10:22:28Z
dc.date.available2017-07-06T10:22:28Z
dc.date.issued2015-08-12
dc.identifier.citationHorzum, U., Özdil, B., and Pesen Okvur, D. (2015). Differentiation of normal and cancer cell adhesion on custom designed protein nanopatterns. Nano Letters, 15(8), 5393-5406. doi:10.1021/acs.nanolett.5b01785en_US
dc.identifier.issn1530-6984
dc.identifier.urihttps://doi.org/10.1021/acs.nanolett.5b01785
dc.identifier.urihttp://hdl.handle.net/11147/5871
dc.description.abstractCell adhesion to the extracellular matrix is deregulated in metastasis. However, traditional surfaces used to study cell adhesion do not faithfully mimic the in vivo microenvironment. Electron beam lithography (EBL) is able to generate customized protein nanopatterns. Here, we used an EBL-based green lithography approach to fabricate homogeneous and gradient, single (fibronectin, K-casein) and double (fibronectin, laminin) active component protein nanopatterns with micrometer scale spacing to investigate differences in adhesion of breast cancer cells (BCC) and normal mammary epithelial cells (NMEC). Our results showed that as expected, in contrast to NMEC, BCC were plastic: they tolerated nonadhesion promoting regions, adapted to flow and exploited gradients better. In addition, the number of focal adhesions but not their area appeared to be the dominant parameter for regulation of cell adhesion. Our findings also demonstrated that custom designed protein nanopatterns, which can properly mimic the in vivo microenvironment, enable realistic distinction of normal and cancerous cell adhesion.en_US
dc.description.sponsorshipTUBITAK (111T026); DPT (2009K120860)en_US
dc.language.isoengen_US
dc.publisherAmerican Chemical Societyen_US
dc.relationinfo:eu-repo/grantAgreement/TUBITAK/TBAG/111T026en_US
dc.relation.isversionof10.1021/acs.nanolett.5b01785en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectElectron beam lithographyen_US
dc.subjectCanceren_US
dc.subjectFibronectinen_US
dc.subjectFocal adhesionen_US
dc.subjectLamininen_US
dc.subjectNanopatternen_US
dc.titleDifferentiation of normal and cancer cell adhesion on custom designed protein nanopatternsen_US
dc.typearticleen_US
dc.contributor.authorIDTR114932en_US
dc.contributor.iztechauthorHorzum, Utku
dc.contributor.iztechauthorÖzdil, Berrin
dc.contributor.iztechauthorPesen Okvur, Devrim
dc.relation.journalNano Lettersen_US
dc.contributor.departmentİYTE, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.identifier.volume15en_US
dc.identifier.issue8en_US
dc.identifier.startpage5393en_US
dc.identifier.endpage5403en_US
dc.identifier.wosWOS:000359613700082
dc.identifier.scopusSCOPUS:2-s2.0-84939173235
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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