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dc.contributor.authorDemir, Ayşe Banu
dc.contributor.authorKoç, Ahmet
dc.date.accessioned2017-06-30T11:20:03Z
dc.date.available2017-06-30T11:20:03Z
dc.date.issued2015-12
dc.identifier.citationDemir, A.B., and Koç, A. (2015). High-copy overexpression screening reveals PDR5 as the main doxorubicin resistance gene in yeast. PLoS ONE, 10-12. doi:10.1371/journal.pone.0145108en_US
dc.identifier.issn1932-6203
dc.identifier.urihttps:/doi.org/10.1371/journal.pone.0145108
dc.identifier.urihttp://hdl.handle.net/11147/5822
dc.description.abstractDoxorubicin is one of the most potent anticancer drugs used in the treatment of various cancer types. The efficacy of doxorubicin is influenced by the drug resistance mechanisms and its cytotoxicity. In this study, we performed a high-copy screening analysis to find genes that play a role in doxorubicin resistance and found several genes (CUE5, AKL1, CAN1, YHR177W and PDR5) that provide resistance. Among these genes, overexpression of PDR5 provided a remarkable resistance, and deletion of it significantly rendered the tolerance level for the drug. Q-PCR analyses suggested that transcriptional regulation of these genes was not dependent on doxorubicin treatment. Additionally, we profiled the global expression pattern of cells in response to doxorubicin treatment and highlighted the genes and pathways that are important in doxorubicin tolerance/toxicity. Our results suggest that many efflux pumps and DNA metabolism genes are upregulated by the drug and required for doxorubicin tolerance.en_US
dc.description.sponsorshipTUBITAK (114Z701)en_US
dc.language.isoengen_US
dc.publisherPublic Library of Scienceen_US
dc.relationinfo:eu-repo/grantAgreement/TUBITAK/KBAG/114Z701en_US
dc.relation.isversionof10.1371/journal.pone.0145108en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectDoxorubicinen_US
dc.subjectGenomic DNAen_US
dc.subjectMessenger RNAen_US
dc.subjectPDR5 proteinen_US
dc.subjectSaccharomyces cerevisiaeen_US
dc.titleHigh-copy overexpression screening reveals PDR5 as the main doxorubicin resistance gene in yeasten_US
dc.typearticleen_US
dc.contributor.iztechauthorDemir, Ayşe Banu
dc.contributor.iztechauthorKoç, Ahmet
dc.relation.journalPLoS ONEen_US
dc.contributor.departmentİYTE, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.identifier.volume10en_US
dc.identifier.issue12en_US
dc.identifier.wosWOS:000367092300036
dc.identifier.scopusSCOPUS:2-s2.0-84956921472
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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