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dc.contributor.authorHeerd, Doreen
dc.contributor.authorTarı, Canan
dc.contributor.authorFernandez Lahore, Marcelo
dc.date.accessioned2017-05-31T06:33:32Z
dc.date.available2017-05-31T06:33:32Z
dc.date.issued2014
dc.identifier.citationHeerd, D., Tarı, C., and Fernandez Lahore, M. (2014). Microbial strain improvement for enhanced polygalacturonase production by Aspergillus sojae. Applied Microbiology and Biotechnology, 98(17), 7471-7481. doi:10.1007/s00253-014-5657-zen_US
dc.identifier.issn0175-7598
dc.identifier.urihttps://doi.org/10.1007/s00253-014-5657-z
dc.identifier.urihttp://hdl.handle.net/11147/5652
dc.description.abstractStrain improvement is a powerful tool in commercial development of microbial fermentation processes. Strains of Aspergillus sojae which were previously identified as polygalacturonase producers were subjected to the cost-effective mutagenesis and selection method, the so-called random screening. Physical (ultraviolet irradiation at 254 nm) and chemical mutagens (N-methyl-N′-nitro-N-nitrosoguanidine) were used in the development and implementation of a classical mutation and selection strategy for the improved production of pectic acid-degrading enzymes. Three mutation cycles of both mutagenic treatments and also the combination of them were performed to generate mutants descending from A. sojae ATCC 20235 and mutants of A. sojae CBS 100928. Pectinolytic enzyme production of the mutants was compared to their wild types in submerged and solid-state fermentation. Comparing both strains, higher pectinase activity was obtained by A. sojae ATCC 20235 and mutants thereof. The highest polygalacturonase activity (1,087.2±151.9 U/g) in solid-state culture was obtained by mutant M3, which was 1.7 times increased in comparison to the wild strain, A. sojae ATCC 20235. Additional, further mutation of mutant M3 for two more cycles of treatment by UV irradiation generated mutant DH56 with the highest polygalacturonase activity (98.8±8.7 U/mL) in submerged culture. This corresponded to 2.4-fold enhanced polygalacturonase production in comparison to the wild strain. The results of this study indicated the development of a classical mutation and selection strategy as a promising tool to improve pectinolytic enzyme production by both fungal strains.en_US
dc.description.sponsorshipJacobs University Bremen gGmbH; European Union (PGSYS/ETB-2008-44)en_US
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.relation.isversionof10.1007/s00253-014-5657-zen_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAspergillus sojaeen_US
dc.subjectMutagenesisen_US
dc.subjectPectinaseen_US
dc.subjectPolygalacturonaseen_US
dc.titleMicrobial strain improvement for enhanced polygalacturonase production by Aspergillus sojaeen_US
dc.typearticleen_US
dc.contributor.iztechauthorTarı, Canan
dc.relation.journalApplied Microbiology and Biotechnologyen_US
dc.contributor.departmentİYTE, Mühendislik Fakültesi, Gıda Mühendisliği Bölümüen_US
dc.identifier.volume98en_US
dc.identifier.issue17en_US
dc.identifier.startpage7471en_US
dc.identifier.endpage7481en_US
dc.identifier.wosWOS:000340837600016
dc.identifier.scopusSCOPUS:2-s2.0-84906948085
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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