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dc.contributor.authorÇelik, İbrahim
dc.contributor.authorGültekin, Visam
dc.contributor.authorAllmer, Jens
dc.contributor.authorDoğanlar, Sami
dc.contributor.authorFrary, Anne
dc.date.accessioned2017-05-18T07:10:22Z
dc.date.available2017-05-18T07:10:22Z
dc.date.issued2014-08
dc.identifier.citationÇelik, İ., Gültekin, V., Allmer, J., Doğanlar, S. , and Frary, A. (2014). Development of genomic simple sequence repeat markers in opium poppy by next-generation sequencing. Molecular Breeding, 34(2), 323-334. doi:10.1007/s11032-014-0036-0en_US
dc.identifier.issn1380-3743
dc.identifier.urihttp://doi.org/10.1007/s11032-014-0036-0
dc.identifier.urihttp://hdl.handle.net/11147/5548
dc.description.abstractOpium poppy (Papaver somniferum L.) is an important pharmaceutical crop with very few genetic marker resources. To expand these resources, we sequenced genomic DNA using pyrosequencing technology and examined the DNA sequences for simple sequence repeats (SSRs). A total of 1,244,412 sequence reads were obtained covering 474 Mb. Approximately half of the reads (52 %) were assembled into 166,724 contigs representing 105 Mb of the opium poppy genome. A total of 23,283 non-redundant SSRs were identified in 18,944 contigs (11.3 % of total contigs). Trinucleotide and tetranucleotide repeats were the most abundant SSR repeats, accounting for 49.0 and 27.9 % of all SSRs, respectively. The AAG/TTC repeat was the most abundant trinucleotide repeat, representing 19.7 % of trinucleotide repeats. Other SSR repeat types were AT-rich. A total of 23,126 primer pairs (98.7 % of total SSRs) were designed to amplify SSRs. Fifty-three genomic SSR markers were tested in 37 opium poppy accessions and seven Papaver species for determination of polymorphism and transferability. Intraspecific polymorphism information content (PIC) values of the genomic SSR markers were intermediate, with an average 0.17, while the interspecific average PIC value was slightly higher, 0.19. All markers showed at least 88 % transferability among related species. This study increases sequence coverage of the opium poppy genome by sevenfold and the number of opium poppy-specific SSR markers by sixfold. This is the first report of the development of genomic SSR markers in opium poppy, and the genomic SSR markers developed in this study will be useful in diversity, identification, mapping and breeding studies in opium poppy.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (TUBITAK-109O797)en_US
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.relationinfo:eu-repo/grantAgreement/TUBITAK/TOVAG/109O797en_US
dc.relation.isversionof10.1007/s11032-014-0036-0en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGenetic diversityen_US
dc.subjectGenomic SSRsen_US
dc.subjectGS-FLX pyrosequencing technologyen_US
dc.subjectMicrosatellitesen_US
dc.subjectPapaver somniferum L.en_US
dc.titleDevelopment of genomic simple sequence repeat markers in opium poppy by next-generation sequencingen_US
dc.typearticleen_US
dc.contributor.authorIDTR131451en_US
dc.contributor.authorIDTR107974en_US
dc.contributor.authorIDTR1977en_US
dc.contributor.authorIDTR114553en_US
dc.contributor.iztechauthorÇelik, İbrahim
dc.contributor.iztechauthorGültekin, Visam
dc.contributor.iztechauthorAllmer, Jens
dc.contributor.iztechauthorDoğanlar, Sami
dc.contributor.iztechauthorFrary, Anne
dc.relation.journalMolecular Breedingen_US
dc.contributor.departmentİYTE, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.identifier.volume34en_US
dc.identifier.issue2en_US
dc.identifier.startpage323en_US
dc.identifier.endpage334en_US
dc.identifier.wosWOS:000342164700005
dc.identifier.scopusSCOPUS:2-s2.0-84893180996
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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