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dc.contributor.authorHorzum, Utku
dc.contributor.authorÖzdil, Berrin
dc.contributor.authorPesen Okvur, Devrim
dc.date.accessioned2017-05-05T07:14:58Z
dc.date.available2017-05-05T07:14:58Z
dc.date.issued2014-06
dc.identifier.citationHorzum, U., Özdil, B., and Pesen Okvur, D. (2014). Micrometer scale spacings between fibronectin nanodots regulate cell morphology and focal adhesions. Materials Research Express, 1(2). doi:10.1088/2053-1591/1/2/025402en_US
dc.identifier.issn2053-1591
dc.identifier.urihttps://doi.org/10.1088/2053-1591/1/2/025402
dc.identifier.urihttp://hdl.handle.net/11147/5448
dc.description.abstractCell adhesion to extracellular matrix is an important process for both health and disease states. Surface protein patterns that are topographically flat, and do not introduce other chemical, topographical or rigidity related functionality and, more importantly, that mimic the organization of the in vivo extracellular matrix are desired. Previous work showed that vinculin and cytoskeletal organization are modulated by size and shape of surface nanopatterns. However, quantitative analysis on cell morphology and focal adhesions as a function of micrometer scale spacings of FN nanopatterns was absent. Here, electron beam lithography was used to pattern fibronectin nanodots with micrometer scale spacings on a Kcasein background on indium tin oxide coated glass which, unlike silicon, is transparent and thus suitable for many light microscopy techniques. Exposure times were significantly reduced using the line exposure mode with micrometer scale step sizes. Micrometer scale spacings of 2, 4 and 8 m between fibronectin nanodots proved to modulate cell adhesion through modification of cell area, focal adhesion number, size and circularity. Overall, cell behavior was shown to shift at the apparent threshold of 4 m spacing. The findings presented here offer exciting new opportunities for cell biology research.en_US
dc.description.sponsorshipTUBITAK (111T026); DPT (2009K120860)en_US
dc.language.isoengen_US
dc.publisherIOP Publishingen_US
dc.relation.isversionof10.1088/2053-1591/1/2/025402en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectCell shapeen_US
dc.subjectElectron beam lithographyen_US
dc.subjectFibronectinen_US
dc.subjectFocal adhesionen_US
dc.titleMicrometer scale spacings between fibronectin nanodots regulate cell morphology and focal adhesionsen_US
dc.typearticleen_US
dc.contributor.authorIDTR114932en_US
dc.contributor.iztechauthorHorzum, Utku
dc.contributor.iztechauthorÖzdil, Berrin
dc.contributor.iztechauthorPesen Okvur, Devrim
dc.relation.journalMaterials Research Expressen_US
dc.contributor.departmentİYTE, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.identifier.volume1en_US
dc.identifier.issue2en_US
dc.identifier.wosWOS:000209665000051
dc.identifier.scopusSCOPUS:2-s2.0-84945246058
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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