Show simple item record

dc.contributor.authorAras, Nadir
dc.contributor.authorYalçın, Şerife
dc.date.accessioned2017-04-26T12:18:53Z
dc.date.available2017-04-26T12:18:53Z
dc.date.issued2014-03
dc.identifier.citationAras, N., and Yalçın, Ş. (2014). Rapid identification of phosphorus containing proteins in electrophoresis gel spots by Laser-Induced Breakdown Spectroscopy, LIBS. Journal of Analytical Atomic Spectrometry, 29(3), 545-552. doi:10.1039/c3ja50225ben_US
dc.identifier.issn0267-9477
dc.identifier.urihttp://doi.org/10.1039/c3ja50225b
dc.identifier.urihttp://hdl.handle.net/11147/5411
dc.description.abstractA novel method for the rapid in-gel identification of phosphorus containing proteins, specifically casein and ovalbumin, prior to mass spectrometric analysis for the elucidation of phosphorylation sites was developed. After polyacrylamide gel-electrophoretic separation, staining and drying, protein bands were subjected to focused laser pulses at the center or the vicinity of the protein band. Phosphorus containing proteins were recognized from their prominent phosphorus lines in the luminous plasma formed by energetic laser pulses. The LIBS emission intensity of phosphorus lines at 253.5 nm and 255.3 nm has been optimized with respect to laser energy and detector timing parameters by using pure casein in the pellet form. The method was applied to casein, ovalbumin, two commercially available standard protein mixtures and proteins extracted from the canola plant. It was shown that LIBS was capable of identifying phosphorus containing proteins directly in the gel matrix in nanogram amounts. Mass spectrometric analysis of the ovalbumin spot after the in-gel digestion procedure has proved the accuracy of the technique. With the speed and spatial resolution that LIBS offers, this technique shows promise in the micro-local spotting of phosphorus containing proteins in the polyacrylamide gel matrix prior to MS analysis for the determination of the phosphorylation sites. © 2014 The Royal Society of Chemistry.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey (105T134-108T376-109T327); Izmir Institute of Technologyen_US
dc.language.isoengen_US
dc.publisherRoyal Society of Chemistryen_US
dc.relationinfo:eu-repo/grantAgreement/TUBITAK/TBAG/105T134en_US
dc.relationinfo:eu-repo/grantAgreement/TUBITAK/TBAG/108T376en_US
dc.relationinfo:eu-repo/grantAgreement/TUBITAK/TBAG/109T327en_US
dc.relation.isversionof10.1039/c3ja50225ben_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectProteinsen_US
dc.subjectAtomic emission spectroscopyen_US
dc.subjectElectrophoresisen_US
dc.subjectPhosphorylationen_US
dc.subjectMass spectrometersen_US
dc.subjectTiming parametersen_US
dc.titleRapid identification of phosphorus containing proteins in electrophoresis gel spots by Laser-Induced Breakdown Spectroscopy, LIBSen_US
dc.typereviewen_US
dc.contributor.authorIDTR57067en_US
dc.contributor.authorIDTR114593en_US
dc.contributor.iztechauthorAras, Nadir
dc.contributor.iztechauthorYalçın, Şerife
dc.relation.journalJournal of Analytical Atomic Spectrometryen_US
dc.contributor.departmentİYTE, Fen Fakültesi, Kimya Bölümüen_US
dc.identifier.volume29en_US
dc.identifier.issue3en_US
dc.identifier.startpage545en_US
dc.identifier.endpage552en_US
dc.identifier.wosWOS:000332495200017
dc.identifier.scopusSCOPUS:2-s2.0-84894141478


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record