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dc.contributor.authorErbaşol, Işıl
dc.contributor.authorBozdağ, Gönensin Ozan
dc.contributor.authorKoç, Ahmet
dc.contributor.authorPedas, Pia
dc.contributor.authorKarakaya, Hüseyin Çağlar
dc.date.accessioned2017-03-29T06:34:42Z
dc.date.available2017-03-29T06:34:42Z
dc.date.issued2013
dc.identifier.citationErbaşol, I., Bozdağ, G.O., Koç, A., Pedas, P., and Karakaya, H.Ç. (2013). Characterization of two genes encoding metal tolerance proteins from Beta vulgaris subspecies maritima that confers manganese tolerance in yeast. BioMetals, 26(5), 795-804. doi:10.1007/s10534-013-9658-7en_US
dc.identifier.issn0966-0844
dc.identifier.urihttps://doi.org/10.1007/s10534-013-9658-7
dc.identifier.urihttp://hdl.handle.net/11147/5169
dc.description.abstractManganese (Mn2+) is an essential micronutrient in plants. However increased Mn2+ levels are toxic to plant cells. Metal tolerance proteins (MTPs), member of cation diffusion facilitator protein (CDF) family, have important roles in metal homeostatis in different plant species and catalyse efflux of excess metal ions. In this study, we identified and characterized two MTP genes from Beta vulgaris spp. maritima (B. v. ssp. maritima). Overexpression of these two genes provided Mn tolerance in yeast cells. Sequence analyses displayed BmMTP10 and BmMTP11as members of the Mn-CDF family. Functional analyses of these proteins indicated that they are specific to Mn2+ with a role in reducing excess cellular Mn2+ levels when expressed in yeast. GFP-fusion constructs of both proteins localized to the Golgi apparatus as a punctuated pattern. Finally, Q-RT-PCR results showed that BmMTP10 expression was induced threefold in response to the excess Mn2+ treatment. On the other hand BmMTP11 expression was not affected in response to excess Mn2+ levels. Thus, our results suggest that the BmMTP10 and BmMTP11 proteins from B. v. ssp. maritima have non-redundant functions in terms of Mn2+ detoxification with a similar in planta localization and function as the Arabidopsis Mn-CDF homolog AtMTP11 and this conservation shows the evolutionary importance of these vesicular proteins in heavy metal homeostatis among plant species.en_US
dc.description.sponsorshipDanish Strategic Research Council (NUTRIEFFICIENT) (10-093498); Scientific and Technical Research Council of Turkey (113Z401)en_US
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.relation.isversionof10.1007/s10534-013-9658-7en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectGolgien_US
dc.subjectHeavy metal toleranceen_US
dc.subjectManganeseen_US
dc.subjectYeasten_US
dc.subjectMetal tolerance proteinen_US
dc.titleCharacterization of two genes encoding metal tolerance proteins from Beta vulgaris subspecies maritima that confers manganese tolerance in yeasten_US
dc.typearticleen_US
dc.contributor.authorIDTR110769en_US
dc.contributor.authorIDTR24898en_US
dc.contributor.iztechauthorErbaşol, Işıl
dc.contributor.iztechauthorBozdağ, Gönensin Ozan
dc.contributor.iztechauthorKoç, Ahmet
dc.contributor.iztechauthorKarakaya, Hüseyin Çağlar
dc.relation.journalBioMetalsen_US
dc.contributor.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume26en_US
dc.identifier.issue5en_US
dc.identifier.startpage795en_US
dc.identifier.endpage804en_US
dc.identifier.wosWOS:000326926600013
dc.identifier.scopusSCOPUS:2-s2.0-84885484358
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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