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dc.contributor.authorHuang, Xin
dc.contributor.authorSevimli, Sema İlknur
dc.contributor.authorBulmuş, Volga
dc.date.accessioned2017-03-28T08:09:26Z
dc.date.available2017-03-28T08:09:26Z
dc.date.issued2013-10
dc.identifier.citationHuang, X., Sevimli, S. İ., and Bulmuş, V. (2013). PH-labile sheddable block copolymers by RAFT polymerization: Synthesis and potential use as siRNA conjugates. European Polymer Journal, 49(10), 2895-2905. doi:10.1016/j.eurpolymj.2013.03.036en_US
dc.identifier.issn0014-3057
dc.identifier.urihttps://doi.org/10.1016/j.eurpolymj.2013.03.036
dc.identifier.urihttp://hdl.handle.net/11147/5153
dc.description.abstractWell-defined amphiphilic block copolymers composed of hydrophilic and hydrophobic blocks linked through an acid-labile acetal bond were synthesized directly by RAFT polymerization using a new poly(ethylene glycol) (PEG) macroRAFT agent modified with an acid-labile group at its R-terminal. The new macroRAFT agent was used for polymerization of poly(t-butyl methacrylate) (PtBMA) or poly(cholesterol-methacrylate) (PCMA) to synthesize well-defined block copolymers with a PEG block sheddable under acidic conditions. The chain extension polymerization kinetics showed known traits of RAFT polymerization. The molecular weight distributions of the copolymers prepared using the new macroRAFT agent remained below 1.2 during the polymerizations and the molecular weight of the copolymers was linearly proportional to monomer conversions. The acid-catalyzed hydrolysis behavior of the PEG-macroRAFT agent and the PEG-b-PtBMA (Mn = 13,600 by GPC, PDI = 1.10) was studied by GPC, 1H NMR and UV-vis spectroscopy. The half-life of acid-hydrolysis was 70 min at pH 2.2 and 92 h at pH 4.0. The potential use of the pH-labile shedding behavior of the copolymers was demonstrated by conjugating a thiol-modified siRNA to ω-pyridyldisulfide modified PEG-b-PCMA. The resultant PEG-b-PCMA-b-siRNA triblock modular polymer released PCMA-b-siRNA segment in acidic and siRNA segment in reductive conditions, as confirmed by polyacrylamide gel electrophoresis.en_US
dc.description.sponsorshipAustralian Research Council (ARC) (DP 0770818)en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/j.eurpolymj.2013.03.036en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectBioconjugateen_US
dc.subjectBlock copolymeren_US
dc.subjectDrug deliveryen_US
dc.subjectpH-labileen_US
dc.subjectRAFT polymerizationen_US
dc.subjectsiRNA deliveryen_US
dc.subjectPolyethylene glycolsen_US
dc.titlePH-labile sheddable block copolymers by RAFT polymerization: Synthesis and potential use as siRNA conjugatesen_US
dc.typearticleen_US
dc.contributor.authorIDTR181383en_US
dc.contributor.iztechauthorBulmuş, Volga
dc.relation.journalEuropean Polymer Journalen_US
dc.contributor.departmentİYTE, Mühendislik Fakültesi, Kimya Mühendisliği Bölümüen_US
dc.identifier.volume49en_US
dc.identifier.issue10en_US
dc.identifier.startpage2895en_US
dc.identifier.endpage2905en_US
dc.identifier.wosWOS:000325233800006
dc.identifier.scopusSCOPUS:2-s2.0-84883884021
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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