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dc.contributor.authorHacıoğlu, Elise
dc.contributor.authorDemir, Ayşe Banu
dc.contributor.authorKoç, Ahmet
dc.date.accessioned2017-02-09T08:08:14Z
dc.date.available2017-02-09T08:08:14Z
dc.date.issued2012-02
dc.identifier.citationHacıoğlu, E., Demir, A. B., and Koç, A. (2012). Identification of respiratory chain gene mutations that shorten replicative life span in yeast. Experimental Gerontology, 47(2), 149-153. doi:10.1016/j.exger.2011.11.009en_US
dc.identifier.issn0531-5565
dc.identifier.urihttp://doi.org/10.1016/j.exger.2011.11.009
dc.identifier.urihttp://hdl.handle.net/11147/4816
dc.description.abstractAging is the progressive accumulation of alterations in cells that elevates the risk of death. The mitochondrial theory of aging postulates that free radicals produced by the mitochondrial respiratory system contribute to the aging process. However, the roles of individual electron transfer chain (ETC) components in cellular aging have not been elucidated. In this study, we analyzed the replicative life span of 73 yeast deletion mutants lacking the genes of the mitochondrial electron transfer chain system, and found that nine of these mutants (δ nde1, δ tcm62, δ rip1, δ cyt1, δ qrc8, δ pet117, δ cox11, δ atp11, δ fmc1) had significantly shorter life spans. These mutants had lower rates of respiration and were slightly sensitive to exogenous administration of hydrogen peroxide. However, only two of them, δ nde1 and δ fmc1, produced higher amounts of intrinsic superoxide radicals in the presence of glucose compared to that of wild type cells. Interestingly, there were no significant alterations in the mitochondrial membrane potentials of these mutants. We speculate that the shorter life spans of ETC mutants result from multiple mechanisms including the low respiration rate and low energy production rather than just a ROS-dependent path. © 2011 Elsevier Inc.en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/j.exger.2011.11.009en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAgingen_US
dc.subjectYeasten_US
dc.subjectChronological agingen_US
dc.subjectElectron transport chainen_US
dc.subjectReactive oxygen speciesen_US
dc.subjectReplicative life spanen_US
dc.subjectMitochondriaen_US
dc.titleIdentification of respiratory chain gene mutations that shorten replicative life span in yeasten_US
dc.typearticleen_US
dc.contributor.authorIDTR190238en_US
dc.contributor.authorIDTR110769en_US
dc.contributor.iztechauthorHacıoğlu, Elise
dc.contributor.iztechauthorDemir, Ayşe Banu
dc.contributor.iztechauthorKoç, Ahmet
dc.relation.journalExperimental Gerontologyen_US
dc.contributor.departmentİYTE, Fen Fakültesi, Moleküler Biyoloji ve Genetik Bölümüen_US
dc.identifier.volume47en_US
dc.identifier.issue2en_US
dc.identifier.startpage149en_US
dc.identifier.endpage153en_US
dc.identifier.wosWOS:000300918500004
dc.identifier.scopusSCOPUS:2-s2.0-84856223482
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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