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dc.contributor.authorHarsa, Hayriye Şebnem
dc.contributor.authorFurusaki, Shintaro
dc.date.accessioned2016-05-03T08:46:19Z
dc.date.available2016-05-03T08:46:19Z
dc.date.issued2001-10
dc.identifier.citationHarsa, H. Ş., and Furusaki, S. (2001). Chromatographic separation of amyloglucosidase from the mixtures of enzymes. Biochemical Engineering Journal, 8(3), 257-261. doi:10.1016/S1369-703X(01)00123-1en_US
dc.identifier.issn1369-703X
dc.identifier.urihttp://doi.org/doi:10.1016/S1369-703X(01)00123-1
dc.identifier.urihttp://hdl.handle.net/11147/4590
dc.description.abstractAn affinity chromatographic separation of amyloglucosidase (AMG) enzyme using β-cyclodextrin-chitosan matrix was investigated in this study. The adsorbent selectively adsorbed AMG from the mixtures of enzymes (lactase and α-amylase), and this selectively bound AMG was eluted from the column in two steps with a recovery yield of about 72%. A small fraction of AMG and other contaminating proteins did not bind to the column specifically and were eluted easily. Purified AMG was visualised on the sodiumdodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) experimentsen_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/S1369-703X(01)00123-1en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectAffinityen_US
dc.subjectBioseparationsen_US
dc.subjectChromatographyen_US
dc.subjectGlucoamylaseen_US
dc.subjectBeta cyclodextrinen_US
dc.titleChromatographic separation of amyloglucosidase from the mixtures of enzymesen_US
dc.typearticleen_US
dc.contributor.authorIDTR9626en_US
dc.contributor.iztechauthorHarsa, Hayriye Şebnem
dc.relation.journalBiochemical Engineering Journalen_US
dc.contributor.departmentIzmir Institute of Technology. Food Engineeringen_US
dc.identifier.volume8en_US
dc.identifier.issue3en_US
dc.identifier.startpage257en_US
dc.identifier.endpage261en_US
dc.identifier.wosWOS:000171783800012
dc.identifier.scopusSCOPUS:2-s2.0-0034759362
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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