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dc.contributor.authorGüneş, Hatice
dc.contributor.authorMastro, Andrea M.
dc.date.accessioned2016-03-30T08:41:04Z
dc.date.available2016-03-30T08:41:04Z
dc.date.issued1996-03
dc.identifier.citationGüneş, H., and Mastro, A. M. (1996). Prolactin receptor gene expression in rat splenocytes and thymocytes from birth to adulthood. Molecular and Cellular Endocrinology, 117(1), 41-52. doi:10.1016/0303-7207(95)03724-1en_US
dc.identifier.urihttp://doi.org/10.1016/0303-7207(95)03724-1
dc.identifier.urihttp://hdl.handle.net/11147/4493
dc.description.abstractIn vivo and in vitro studies have indicated that the anterior pituitary hormone prolactin (PRL) is an immunoregulator and functions in the development of the neonatal immune system. In this study, prolactin receptor (PRL-R) expression from birth to adulthood as well as the effect of milk ingestion on the PRL-R expression were examined in splenocytes and thymocytes of neonatal rats. Three approaches were taken to measure PRL-R expression: (i) polymerase chain reaction (RT-PCR); (ii) antibody to PRL-R and Western blotting; (iii) antibody to PRL-R and flow cytometry. RT-PCR analysis revealed the short and long form of PRL-R mRNA in both spleen and thymus at every age tested. However, the long form of PRL-R mRNA was always more abundant than that of the short form. In addition, antipeptide antibody against the long form of PRL-R recognized 84 and 42 kD proteins in the spleen, but only the 84 kD protein in the thymus. A monoclonal antibody U6 recognized 38 and 40 kD proteins in both the spleen and thymus. Although the mRNA level of PRL-R was relatively low at birth and increased with age in both the spleen and thymus, the levels of protein bands detected with both antibodies correlated with development in the spleen; whereas the levels remained steady in the thymus. Therefore, we concluded that the expression of PRL-R at the protein level is developmentally regulated in the spleen but not in the thymus. Finally, milk ingestion in the first seven hours decreased the percentage of cells expressing cell surface PRL-R, suggesting that milk-borne PRL may have a direct effect on lymphocytes.en_US
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/0303-7207(95)03724-1en_US
dc.rightsinfo:eu-repo/semantics/openAccessen_US
dc.subjectMilken_US
dc.subjectNeonatal immunityen_US
dc.subjectProlactinen_US
dc.subjectProlactin receptoren_US
dc.subjectSplenocytesen_US
dc.subjectThymocytesen_US
dc.titleProlactin receptor gene expression in rat splenocytes and thymocytes from birth to adulthooden_US
dc.typearticleen_US
dc.contributor.authorIDTR2082en_US
dc.contributor.iztechauthorGüneş, Hatice
dc.relation.journalMolecular and Cellular Endocrinologyen_US
dc.contributor.departmentIzmir Institute of Technology. Molecular Biology and Geneticsen_US
dc.identifier.volume117en_US
dc.identifier.issue1en_US
dc.identifier.startpage41en_US
dc.identifier.endpage52en_US
dc.identifier.wosWOS:A1996UB08400005
dc.identifier.scopusSCOPUS:2-s2.0-8044249397
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US


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